- Untargeted Metabolomics
- Lipidomics
- Targeted Metabolomics
- Functional Metabolomics
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Proteomics
- Nanoparticle proteomics
- iTRAQ/TMT-based Proteomics Analysis
- Label free Quantitative Proteomics
- Protein Identification
- DIA proteomics
- Peptidomics
- Parallel Reaction Monitoring (PRM) Targeted Proteome
- Metpro -Ⅱ Protein-Metabolite Interactions
- Phosphoproteomics
- Acetylation Analysis
- Protein Ubiquitination Analysis
iTRAQ reagent contains three regions, namely a mass reporter group, a mass balancer group and a protein reactive group N-hydroxysuccinimide ester (NHS) that introduces a highly basic group at lysine side chains and at peptide N-termini (Figure 3). The first type of iTRAQ reagent, a 4-plex has the variable mass between 114–117 Da within the reporter group.The tandem mass tags (TMTs) are chemical labeling reagents commonly used for mass spectrometry-based quantification and identification of peptides and proteins. The idea of TMTs is based on a similar principle in comparison to iTRAQ, with up to six possible labels. The TMT reagent, proposed by Thompson et al. in 2003, is composed of a mass normalization group (balancegroup) that balances mass differences from individual reporter ions to ensure the same overall mass of the reagents, a reporter group that provides the abundance of a peptide upon MS/MS in individual samples being mixed, and an amino group reactive functionality making the modification of primary amines possible.The iTRAQ and other isobaric-tag labeling are performed on digested protein; therefore, every peptide formed during proteolysis should be labeled. The detection of multiple peptides originating from one protein may be achieved providing multiple quantitation per protein. This feature makes possible the identification and quantification of low-abundance proteins in complex samples. 。
Advantages
Wide range of application: in addition to cytoplasmic proteins, detectable proteins also include mitochondrial proteins, membrane proteins and nuclear proteins.
High throughput: it can realize the qualitative and quantitative analysis of multiple samples at one time.
The results are reliable: Based on high sensitivity and high resolution tandem mass spectrometry, the correlation of protein expression between repeated samples is high.
High sensitivity: Fractionation reduces sample complexity.
Strong separation ability: it can separate acid / basic proteins, proteins less than 10kDa or greater than 200kDa.
Application
Disease biomarkers
Molecular mechanism of occurrence and development of diseases
Target of chemical or biological drugs
Action mechanism and signal transduction of chemical or biological drugs
Animal husbandry: improving meat quality and feeding conditions
Agriculture: crop development mechanism
Food Science:improving food safety, taste and nutrition
