- Untargeted Metabolomics
- Lipidomics
- Targeted Metabolomics
- Functional Metabolomics
-
Proteomics
- Nanoparticle proteomics
- iTRAQ/TMT-based Proteomics Analysis
- Label free Quantitative Proteomics
- Protein Identification
- DIA proteomics
- Peptidomics
- Parallel Reaction Monitoring (PRM) Targeted Proteome
- Metpro -Ⅱ Protein-Metabolite Interactions
- Phosphoproteomics
- Acetylation Analysis
- Protein Ubiquitination Analysis
Oxidized lipids, derived from oxygenated polyunsaturated fatty acids and originating from the acyl groups of galactolipids in chloroplast membranes, play a crucial role in plant growth, development, and responses to pathogens and abiotic stress. Plant lipoxygenases (LOX) catalyze the dioxygenation of linoleic acid (C18:2) and linolenic acid (C18:3), forming corresponding hydroperoxy fatty acids (Fatty Acid Synthase, FAs), which are involved in the synthesis of oxidized lipids. These oxidized lipids participate in stress signal transduction, regulating stress-induced gene expression, and interact with other signaling pathways in plant cells, including those mediated by plant hormones such as auxins, gibberellins, ethylene, and abscisic acid.
Oxidized lipid reactions involve the automatic oxidation of polyunsaturated fatty acids or the enzymatic catalysis leading to the generation of a series of oxidized metabolites, referred to as lipid mediators. These lipid mediators play vital roles in various biological processes, including inflammation, immune defense, endocrine regulation, and metabolic stress. Research has revealed that metabolites from oxidized lipid metabolism play important regulatory roles in the occurrence and development of diseases such as tumors, neurodegenerative diseases, cardiovascular diseases, digestive system disorders, and urinary system diseases.
The technical advantages in this field include accurate quantification through isotope internal standard methods, enabling the simultaneous detection of 130+ substances.
Applications:
Cardiovascular diseases
Investigations into drug targets.
Collaboration Case：
Absolute quantification and characterization of oxylipins in lupus nephritis and systemic lupus erythematosus.2022.Frontiers in Immunology.(IF=8.786)[Client paper]
