- Untargeted Metabolomics
- Lipidomics
- Targeted Metabolomics
- Functional Metabolomics
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Proteomics
- Nanoparticle proteomics
- iTRAQ/TMT-based Proteomics Analysis
- Label free Quantitative Proteomics
- Protein Identification
- DIA proteomics
- Peptidomics
- Parallel Reaction Monitoring (PRM) Targeted Proteome
- Metpro -Ⅱ Protein-Metabolite Interactions
- Phosphoproteomics
- Acetylation Analysis
- Protein Ubiquitination Analysis
Label-free protein quantification is a mass spectrometry-based method for identifying and quantifying relative changes in two or more biological samples instead of using a stable isotope-containing compound to label proteins. There is no limit on the number of samples in label-free protein quantification and it’s in principle applicable to any kind of sample, including materials that can not be directly metabolically labeled (for instance, many clinical samples). It is ideal for large-sample analyses in clinical screening or biomarker discovery experiments.
Advantages
The variability that chemical labeling/tagging may introduce is eliminated.
Chemical and metabolic tags are usually expensive.
The time for sample preparation is significantly reduced by the elimination of numerous steps.
Application
Disease biomarkers
Molecular mechanism of occurrence and development of diseases
Target of chemical or biological drugs
Action mechanism and signal transduction of chemical or biological drugs
Animal husbandry: improving meat quality and feeding conditions
Agriculture: crop development mechanism
Food Science:improving food safety, taste and nutrition
